研究方向
黄岩谊课题组主要从事单细胞分析化学、微流控技术、高通量基因组测序技术的研究，并研发新型的生命分析化学仪器。他的研究团队通过融合生物技术、生物物理、计算机、分析化学等多个学科的技术进步，来实现对复杂生物体系的定量和高通量测量。在过去几年，黄岩谊课题组着重发展了纠错编码的新型 DNA 测序策略，提出了新的测序原理和提高测序原始准确率的新方法。同时，针对单细胞定量分析的新方法和新技术，包括在低拷贝数核酸检测上和在单细胞全基因组分析、全转录组分析和极少量细胞表观遗传组分析方面，实现了一系列重要技术突破。他们在集成微流控芯片、单细胞基因组分析技术与器件、单细胞定量化学成像分析方法方面的工作，可以很好地 将表现型和基因型及其动态调控很好的关联起来，在单细胞层次上对复杂体系进行多维度、高精度的测量分析，提供可靠数据并揭示功能相关性。
代表性论文
1. Ruoyan Li,# Lin Di,# Jie Li,# Wenyi Fan,# Yachen Liu, Wenjia Guo, Weiling Liu, Lu Liu, Qiong Li, Liping Chen, Yamei Chen, Chuanwang Miao, Hongjin Liu, Yuqian Wang, Yuling Ma, Deshu Xu, Dongxin Lin*, Yanyi Huang*, Jianbin Wang*, Fan Bai*, Chen Wu*. A body map of somatic mutagenesis in morphologically normal human tissues. Nature, 2021, 597 (7876): 398-403.
2. Lin Di, Yusi Fu, Yue Sun, Jie Li, Lu Liu, Jiacheng Yao, Guanbo Wang, Yalei Wu, Kaiqin Lao, Raymond W Lee, Genhua Zheng, Jun Xu, Juntaek Oh, Dong Wang, X. Sunney Xie*, Yanyi Huang*, Jianbin Wang*. RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids. PNAS, 2020, 117: 2886-2893.
3. Xiannian Zhang, Tianqi Li, Feng Liu, Yaqi Chen, Jiacheng Yao, Zeyao Li, Yanyi Huang*, Jianbin Wang*. Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems. Molecular Cell, 2019, 73: 130-142.
4. Zitian Chen, Wenxiong Zhou, Shuo Qiao, Li Kang, Haifeng Duan, X. Sunney Xie, Yanyi Huang*. Highly accurate fluorogenic DNA sequencing with information theory-based error correction. Nature Biotechnology, 2017, 35(12):1170-78.
5. Lin Di,# Bo Liu,# Yuzhu Lyu, Shihui Zhao, Yuhong Pang, Chen Zhang, Jianbin Wang,* Hai Qi,* Jie Shen,* Yanyi Huang*. SHERRY2: A method for rapid and sensitive single cell RNA-seq. BMC Biology, 2022, 20 213.
6. Xinghuo Pang,# Lili Ren,# Shuangsheng Wu,# Wentai Ma,# Jian Yang, Lin Di, Jie Li, Yan Xiao, Lu Kang, Shichang Du, Jing Du, Jing Wang, Gang Li, Shuguang Zhai, Lijuan Chen, Wenxiong Zhou, Shengjie Lai, Lei Gao, Yang Pan,* Quanyi Wang,* Mingkun Li,* Jianbin Wang,* Yanyi Huang,* Jianwei Wang,* COVID-19 Field Response Group, COVID-19 Laboratory Testing Group. Cold-chain food contamination as the possible origin of Covid-19 resurgence in Beijing. National Science Review, 2020, 7 (12): 1861-1864.
7. Peiyu Liao,# Mengcheng Jiang,# Zitian Chen, Fangli Zhang, Yue Sun, Jun Nie, Meijie Du, Jianbin Wang,* Peng Fei,* Yanyi Huang*. 3D digital PCR through light-sheet imaging of optically cleared emulsion. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (41): 25628-25633.
