研究方向
林一瀚课题组的研究聚焦在单细胞水平上定量解析人类发育、疾病及免疫等重要过程中的基因调控机制，并利用合成生物器件对这些过程实施精准干扰或控制。研究从以下几个方面展开：在单细胞水平上动态解析肿瘤及免疫细胞的状态异质性、表观机制对基因表达和细胞群体异质性的影响、转录因子动力学在信号传导及基因调控中的功能和设计原理、数据驱动的基因调控网络推断及构建，及新型合成生物学器件的设计和应用。
实验室致力于开展前沿的生命科学交叉研究，有机结合了包括系统生物学、合成生物学、定量单细胞分析、活细胞时序荧光成像、数学建模等研究方法。同时，为了引领单细胞研究领域，实验室正在研发高通量、高时空分辨率的单细胞分析工具，用于解析疾病过程中的基因调控网络，并为单细胞水平上药物筛选及大规模测试合成生物学器件等应用提供新平台。
代表性科研论文
1. Lingfeng Xue, Yan Wu, Yihan Lin*. Dissecting and improving gene regulatory network inference using single-cell transcriptome data. Genome Research, 2023, doi:10.1101/gr.277488.122. |
2. Jianhan Zhang, Xu Han, Liang Ma, Shuhui Xu, Yihan Lin*. Deciphering a global source of non-genetic heterogeneity in cancer cells. Nucleic Acids Research, 2023, gkad666. |
3. Wen Huang, Wei Lin, Baoqiang Chen, Jianhan Zhang, Peifen Gao, Yingying Fan, Yihan Lin*, Ping Wei*, NFAT and NF-κB dynamically co-regulate TCR and CAR signaling responses in human T cells, Cell Reports, 2023, 42(7):112663. |
4. Jiegen Wu#, Baoqiang Chen#, Yadi Liu, Liang Ma, Wen Huang, Yihan Lin*, Modulating gene regulation function by chemically controlled transcription factor clustering, Nature Communications, 2022, 13:2663. |
5. Yan Wu#, Lingfeng Xue#, Huang Wen#, Minghua Deng, Yihan Lin*, Profiling transcription factor activity dynamics using intronic reads in time-series transcriptome data, PLOS Computational Biology, 2022, 18(1): e1009762. |
6. Liang Ma, Zeyue Gao, Jiegen Wu, Bijunyao Zhong, Yuchen Xie, Wen Huang, Yihan Lin*. Co-condensation between transcription factor and coactivator p300 modulates transcriptional bursting kinetics. Molecular Cell, 2021, 81:1682-1697. |
7. Yan Wu, Jiaqi Wu, Minghua Deng, Yihan Lin*. Yeast cell fate control by temporal redundancy modulation of transcription factor paralogs. Nature Communications, 2021, 12(1):3145. |
8. Jiegen Wu, Xu Han, Haotian Zhai, Tingyu Yang, Yihan Lin*, Evidence for rate-dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells, Molecular Systems Biology, 2020, 16: e9335. |
9. Jianhan Zhang#, Xu Han#, Yihan Lin*, Dissecting the regulation and function of ATP at the single-cell level, PLOS Biology, 2018, 16(12): e3000095 (Primer). |
10. Bingteng Xie, Yang Pu, Fan Yang, Wei Chen, Wei Yue, Jihong Ma, Na Zhang, Yuening Jiang, Jiegen Wu, Yihan Lin, Xin Liang, Chu Wang, Peng Zou*, Mo Li*, Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification, Cancer Research, 2022, 82 (14): 2576–2592. |
11. Rosa Martinez-Corral, Elba Raimundez, Yihan Lin, Michael B Elowitz, Jordi Garcia-Ojalvo*, Self-amplifying pulsatile protein dynamics without positive feedback, Cell Systems, 2018, 7(4): 453-462. |
12. Jin Park, Marta Dies, Yihan Lin, Sahand Hormoz, Stephanie E. Smith-Unna, Sofia Quinodoz, María Jesús Hernández-Jiménez, Jordi Garcia-Ojalvo#, James C.W. Locke#, Michael B. Elowitz#, Molecular time sharing through dynamic pulsing in single cells, Cell Systems, 2018, 6(2): 216-229. |
13. Yuval Yifat, Nishant Sule, Yihan Lin, Norbert F. Scherer, Analysis and correction of errors in nanoscale particle tracking using the Single-pixel interior filling function (SPIFF) algorithm, Scientific Reports, 2017, 7, 16553. |
# equal contributions
* corresponding author
