研究方向：陈匡时老师致力于活细胞内RNA、DNA以及蛋白质原位可视化技术的开发及生物学应用。2013年4月加入北京大学以来已建立了可实时监测单一RNA与单基因位点动态行为的单分子RNA/DNA活细胞原位可视化技术（fluorescence in vivo hybridization, FIVH）平台。发现了microRNA能够与HIV-1和MLV病毒的结构蛋白Gag互作并干扰影响病毒颗粒的组装，并通过超分辨率荧光显微镜解析了病毒组装过程中HIV-1病毒蛋白Gag与病毒RNA以及宿主细胞蛋白质的互作模式。
代表性科研论文：
1. Qu, N., Ying, Y., Qin, J., and Chen, A.K.* Rational design of self- assembled RNA nanostructures for HIV-1 virus assembly blockade. Nucleic Acids Res.,2022, 50(8): e44
2. Mao, S., Ying, Y., Zhao, M., Yang, Y., and Chen, A.K.* A Background Assessable and Correctable Bimolecular Fluorescence Complementation System for Nanoscopic Single-Molecule Imaging of Intracellular Protein-Protein Interactions. ACS Nano. 2021；15, 14338-14346.
3. Mao, S., Ying, Y., Wu, X., Krueger, C.J. and Chen, A.K.* CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Nucleic Acids Res., 2019; 47, e131.
4. 4. Yang, Y., Qu, N., Tan, J., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* Roles of Gag-RNA interactions in HIV-1 virus assembly deciphered by single-molecule localization microscopy. Proc Natl Acad Sci U S A. 2018; 115, 6721-6726.
5. Wu, X., Mao, S., Yang, Y., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. 2018; Nucleic Acids Res, 46, e80.
6. Qu, N., Ma, Z., Zhang, M., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* Inhibition of retroviral Gag assembly by non-silencing miRNAs promotes autophagic viral degradation. Protein & Cell. 2018; 9, 640-651.
