2018年7月12日,学术期刊《Cell》在线发表北京大学生命科学学院、北大-清华生命科学联合中心、PKU-IDG/麦戈文脑科学研究所李毓龙研究组题为“A genetically-encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice”的研究论文。该研究中,李毓龙研究组开发了新型、可基因编码的多巴胺荧光探针,并将其应用在果蝇、斑马鱼和小鼠中检测内源多巴胺动态变化。该探针将成为研究多巴胺相关神经环路的重要工具。
北京大学生命科学学院李毓龙研究员为本文的通讯作者。李毓龙研究组博士生孙芳妙、曾健智、井淼为共同第一作者;冯杰思、骆奕辰、雍自昊、王欢为此项研究成果做出了重要贡献。该工作的合作者有:中国科学院神经科学研究所的杜久林研究组、徐敏研究组;上海交通大学张思宇研究组;美国国立卫生研究院的崔国红研究组;纽约大学的林大宇研究组;加州大学旧金山分校的Anatol C. Kreitzer研究组。本工作获得了北京大学生物膜与膜生物工程国家重点实验室、北大-清华生命科学联合中心、国家重点基础研究发展计划(973计划)、国家自然科学基金、美国脑计划对李毓龙研究组的大力支持。
1. Holroyd, C. B. & Coles, M. G. H. The neural basis of human error processing: reinforcement learning, dopamine, and the error-related negativity. Psychol Rev 109, 679-709, doi:10.1037/0033-295X.109.4.679 (2002).
2. Schultz, W. Dopamine reward prediction-error signalling: a two-component response. Nature Reviews Neuroscience 17, 183 (2016).
3. Wise, R. A. Dopamine, learning and motivation. Nature reviews neuroscience 5, 483 (2004).
4. Nieoullon, A. Dopamine and the regulation of cognition and attention. Progress in neurobiology 67, 53-83 (2002).
5. Graybiel, A. M., Aosaki, T., Flaherty, A. W. & Kimura, M. The basal ganglia and adaptive motor control. Science 265, 1826-1831 (1994).
6. Cook Jr, E. H. et al. Association of attention-deficit disorder and the dopamine transporter gene. American journal of human genetics 56, 993 (1995).
7. Howes, O. D. & Kapur, S. The dopamine hypothesis of schizophrenia: version III—the final common pathway. Schizophrenia bulletin 35, 549-562 (2009).
8. Lotharius, J. & Brundin, P. Pathogenesis of Parkinson's disease: dopamine, vesicles and α-synuclein. Nature Reviews Neuroscience 3, 932 (2002).
9. Ritz, M. C., Lamb, R. & Kuhar, M. Cocaine receptors on dopamine transporters are related to self-administration of cocaine. Science 237, 1219-1223 (1987).
10. Di Chiara, G. & Imperato, A. Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats. Proc Natl Acad Sci U S A 85, 5274-5278 (1988).
11. Giros, B., Jaber, M., Jones, S. R., Wightman, R. M. & Caron, M. G. Hyperlocomotion and indifference to cocaine and amphetamine in mice lacking the dopamine transporter. Nature 379, 606-612, doi:10.1038/379606a0 (1996).
12. Hernandez, L. & Hoebel, B. G. Food reward and cocaine increase extracellular dopamine in the nucleus accumbens as measured by microdialysis. Life sciences 42, 1705-1712 (1988).